Candida albicans Antimicrobial and Antibiofilm Activity of Novel Endodontic Solvents

Background: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, ability of C. biofilm to tolerate novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially standard irrigation sodium hypochlorite (NaOCl) ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures SC 5314 were treated with NaOCl EDTA exposed MEK/TCE or MEK/OOil. The effect antimicrobial treatment evaluated using disk diffusion method for planktonic culture, enumeration colony-forming units (CFUs) scanning electron microscope (SEM) culture. Results: mature (24 h) significantly reduced biomass viability after mixtures’ exposure, compared previous treatments. MEK/OOil combination caused a total reduction biofilm, while MEK/TCE, there 3-log (CFU/cm2) sequence EDTA, 4-log control. Conclusions: additional exposure preformed 24 h positive antibiofilm impact, overcoming performance conventional irrigating protocol.